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KMID : 0545119990090020163
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 2 p.163 ~ p.167
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Characterization of an Acidic Polysaccharide from Fruiting Bodies of Lyophyllum shimeji
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Lee, Jae Hoon
Cho, Soo Muk/Han, Sang Bae/Kim, Hwan Mook/Yoo, Ick Dong
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Abstract
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An acidic polysaccharide H-Ⅲ was extracted from fruiting bodies of Lyophyllum shimeji with hot water. Acid hydrolysis and gas chromatography analysis showed that the polysaccharide was almost exclusively composed of glucose with a very small amount of mannose and galactose. Uronic acid of 8.36% was also detected in H-Ⅲ. Its molecular weight was estimated to be 1×10exp(6) Da. By ^13C-NMR analysis, some repeating units of disaccharide were detected in the polymer H-Ⅲ. The polysaccharide showed a strong mitogenic activity in a dose-dependent manner.
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